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dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.contributor.authorSanjuan, Antton
dc.contributor.authorBou-Ali, M. Mounir
dc.contributor.otherRivas-Macho, Ane
dc.contributor.otherEletxigerra, Unai
dc.contributor.otherDiez-Ahedob, Ruth
dc.contributor.otherMerino, Santos
dc.contributor.otherRuiz-Rubio, Leire
dc.contributor.otherdel Campo, Javier
dc.contributor.otherVilas-Vilela, José Luis
dc.contributor.otherGoñi-de-Cerio, Felipe
dc.contributor.otherOlabarria, Garbiñe
dc.date.accessioned2023-01-27T11:52:44Z
dc.date.available2023-01-27T11:52:44Z
dc.date.issued2023
dc.identifier.issn2405-8440en
dc.identifier.otherhttps://katalogoa.mondragon.edu/janium-bin/janium_login_opac.pl?find&ficha_no=171372en
dc.identifier.urihttps://hdl.handle.net/20.500.11984/5970
dc.description.abstractThe aim of this work is the design and 3D printing of a new electrochemical sensor for the detection of Listeria monocytogenes based on loop mediated isothermal amplification (LAMP). The food related diseases involve a serious health issue all over the world. Listeria monocytogenes is one of the major problems of contaminated food, this pathogen causes a disease called listeriosis with a high rate of hospitalization and mortality. Having a fast, sensitive and specific detection method for food quality control is a must in the food industry to avoid the presence of this pathogen in the food chain (raw materials, facilities and products). A point-of-care biosensor based in LAMP and electrochemical detection is one of the best options to detect the bacteria on site and in a very short period of time. With the numerical analysis of different geometries and flow rates during sample injection in order to avoid bubbles, an optimized design of the microfluidic biosensor chamber was selected for 3D-printing and experimental analysis. For the electrochemical detection, a novel custom gold concentric-3-electrode consisting in a working electrode, reference electrode and a counter electrode was designed and placed in the bottom of the chamber. The LAMP reaction was optimized specifically for a primers set with a limit of detection of 1.25 pg of genomic DNA per reaction and 100% specific for detecting all 12 Listeria monocytogenes serotypes and no other Listeria species or food-related bacteria. The methylene blue redox-active molecule was tested as the electrochemical transducer and shown to be compatible with the LAMP reaction and very clearly distinguished negative from positive food samples when the reaction is measured at the end-point inside the biosensor.en
dc.description.sponsorshipGobierno Vasco-Eusko Jaurlaritzaes
dc.language.isoengen
dc.publisherElsevieren
dc.rights© 2023 The Authorsen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLoop-mediated isothermal AMPlification (LAMP)en
dc.subjectListeria monocytogenesen
dc.subjectElectrochemical detectionen
dc.subjectBiosensoren
dc.subject3D printingen
dc.subjectNumerical analysisen
dc.titleDesign and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplificationen
dcterms.accessRightshttp://purl.org/coar/access_right/c_abf2en
dcterms.sourceHeliyonen
local.contributor.groupMecánica de fluidoses
local.description.peerreviewedtrueen
local.identifier.doihttps://doi.org/10.1016/j.heliyon.2022.e12637en
local.relation.projectIDinfo:eu-repo/grantAgreement/GV/Elkartek 2021/KK-2021-00082/CAPV/Microtecnologías como motor de desarrollo de Microsistemas avanzados integrados en la Fábrica Inteligente y Digital en el marco de la IIoT4.0/μ4IIOTen
local.relation.projectIDinfo:eu-repo/grantAgreement/GV/Ikertalde Convocatoria 2022-2025/IT1505-22/CAPV/Mecánica de fluidos/en
local.contributor.otherinstitutionBasque Research and Technology Allianceen
local.contributor.otherinstitutionhttps://ror.org/033vryh36eu
local.contributor.otherinstitutionhttps://ror.org/000xsnr85eu
local.source.detailsVol. 9. N. 1. N. artículo e12637en
oaire.format.mimetypeapplication/pdf
oaire.file$DSPACE\assetstore
oaire.resourceTypehttp://purl.org/coar/resource_type/c_6501en
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85en


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Registro sencillo

Attribution-NonCommercial-NoDerivatives 4.0 International
Excepto si se señala otra cosa, la licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 International