2026-04-30T14:21:02Zhttps://ebiltegia.mondragon.edu/oai/requestoai:ebiltegia.mondragon.edu:20.500.11984/58352024-03-01T12:07:51Zcom_20.500.11984_473col_20.500.11984_478
eBiltegia
author
Zabala, Alaitz
other
Lafuente Merchan, M.
other
Ruiz Alonso, S.
other
Espona Noguera, A.
other
Galvez Martin, Patricia
other
López Ruiz, E.
other
Marchal, J.A.
other
López Donaire, M.L.
other
Ciriza, J.
other
Saenz del Burgo, L.
other
Pedraz, J.L.
2022-11-11T09:41:52Z
2022-11-11T09:41:52Z
2021
0928-4931
https://katalogoa.mondragon.edu/janium-bin/janium_login_opac.pl?find&ficha_no=163591
https://hdl.handle.net/20.500.11984/5835
3D-bioprinting is an emerging technology of high potential in tissue engineering (TE), since it shows effective control over scaffold fabrication and cell distribution. Biopolymers such as alginate (Alg), nanofibrillated cellulose (NC) and hyaluronic acid (HA) offer excellent characteristics for use as bioinks due to their excellent biocompatibility and rheological properties. Cell incorporation into the bioink requires sterilisation assurance, and autoclave, β-radiation and γ-radiation are widely used sterilisation techniques in biomedicine; however, their use in 3D-bioprinting for bioinks sterilisation is still in their early stages. In this study, different sterilisation procedures were applied on NC-Alg and NC-Alg-HA bioinks and their effect on several parameters was evaluated. Results demonstrated that NC-Alg and NC-Alg-HA bioinks suffered relevant rheological and physicochemical modifications after sterilisation; yet, it can be concluded that the short cycle autoclave is the best option to sterilise both NC-Alg based cell-free bioinks, and that the incorporation of HA to the NC-Alg bioink improves its characteristics. Additionally, 3D scaffolds were bioprinted and specifically characterized as well as the D1 mesenchymal stromal cells (D1-MSCs) embedded for cell viability analysis. Notably, the addition of HA demonstrates better scaffold properties, together with higher biocompatibility and cell viability in comparison with the NC-Alg scaffolds. Thus, the use of MSCs containing NC-Alg based scaffolds may become a feasible tissue engineering approach for regenerative medicine.
eng
© 2021 Elsevier Ltd
3D-bioprinting
Sterilisation
bioinks
tissue engineering
Development, characterization and sterilisation of Nanocellulose-alginate-(hyaluronic acid)- bioinks and 3D bioprinted scaffolds for tissue engineering
http://purl.org/coar/resource_type/c_6501

EBILTEGIA-N, MONDRAGON UNIBERTSITATEKO GORDAILU IREKIAN, MATERIALAK UZTEKO ERABILERA LIZENTZIA ETA ESKUBIDEEN LAGAPEN EZ-ESKLUSIBOA

Gordailuan utzitako materialaren egile edo egile eskubideen titular naizen aldetik, Mondragon Unibertsitateari erabilera-baimena eta/edo LAGAPEN EZ-ESKLUSIBOA ematen diot, doan, mundu eremuan eta legezko gehieneko epean, material horren gaineko erreprodukzio, banaketa, komunikazio publiko –elektronikoki eskuragarri jartzeko eskubidea barne– eta eraldatzeko eskubideetarako, hirugarren batzuen esku jartzeko aukera emanez, gordailuan utzitako material horri lotutako dagokion lizentzian ezarritako erabilera baldintzen arabera.

ONARTZEN DUT Mondragon Unibertsitateak, edukia aldatu gabe, gordailuan utzitako materiala gordetzeko beharrezkoak diren beste formatu edo bitarteko batzuetara eraldatzea edo egokitzea. Era berean, ONARTZEN DUT Mondragon Unibertsitateak gordailuan utzitako materialaren kopia bat baino gehiago gordetzea, segurtasun kopiak egiteko eta materiala etorkizunerako gordetzeko.

ZIURTATZEN DUT eta BERMATZEN DUT gordailuan utzitako materiala nik sortutako jatorrizko obra dela, eta nik dudala Lizentzia honetan jasotako lagapena emateko beharrezko egile eskubidearen titulartasuna.

Titulartasuna partekatzen den kasuetan, onartzen dut gainerako titularren baimena dudala Lizentzia hau emateko. Eskubideak hirugarren batzuei aurrez lagata izanez gero, ZIURTATZEN dut haien baimena dudala edo lizentzia honetan aurrez ikusitako moduan eskubideak erabiltzeko gaitasuna mantentzen dudala.

Lagatako materialak egile eskubideen titular ez naizen bestelako material batzuk izango balitu, ZIURTATZEN DUT eta BERMATZEN DUT dagokion titularraren baimena lortu dudala aurrez Lizentzia hau eman ahal izateko eta, era berean, ZIURTATZEN DUT eta BERMATZEN dut gordailuan utzitako materialaren edukian behar bezala identifikatuta eta aitortuta dagoela egile eskubidearen titular hori.

Azken batean, ZIURTATZEN DUT eta BERMATZEN DUT eBiltegian gordailuan utzitako materialak EZ DUELA HAUSTEN hirugarren batzuen eskubiderik, ez jabetza intelektualekorik ez industrialekorik, ohore, intimitate eta irudi eskubiderik, edo bestelako edozein eskubiderik.

Gordailuan utzitako materiala Mondragon Unibertsitatea ez den beste agentzia edo erakunderen batek finantzatutako ikerketan oinarrituta baldin badago, ZIURTATZEN DUT eta BERMATZEN DUT bete ditudala erakunde horrekin sinatutako kontratuak edo akordioak eskatutako betebehar guztiak.

Mondragon Unibertsitateak konpromisoa hartzen du gordailuan utzitako materialaren egile edo egile eskubideen titular gisa zu identifikatzeko, eta ez du material horretan Lizentzia honek espresuki ahalbidetutakoa ez den aldaketarik egingo.

Gordailuan utzitako materialaren egile edo egile eskubideen titular zaren heinean, obra eBiltegitik kentzeko eska dezakezu justifikatutako arrazoi batekin. Horretarako, arduradunekin harremanetan jarri beharko duzu.

Era berean, eBiltegiak gordailuan utzitako materiala kendu ahal izango du behar bezala justifikatutako kasuetan edo jabetza intelektualeko eskubideei lotuta hirugarren batzuen erreklamaziorik jasoz gero. Bi kasuetan aurretiaz espresuki jakinaraziko da.


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http://ebiltegia.mondragon.edu/xmlui/bitstream/20.500.11984/5835/1/Development%2c%20characterization%20and%20sterilisation%20of%20Nanocellulose-alginate-%28hyaluronic%20acid%29-%20bioinks%20and%203D%20bioprinted%20scaffolds%20for%20tissue%20engineering.pdf
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Development, characterization and sterilisation of Nanocellulose-alginate-(hyaluronic acid)- bioinks and 3D bioprinted scaffolds for tissue engineering.pdf
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