2026-06-25T19:53:22Zhttps://ebiltegia.mondragon.edu/oai/request

oai:ebiltegia.mondragon.edu:20.500.11984/145952026-06-19T06:15:49Zcom_20.500.11984_473com_20.500.11984_14090col_20.500.11984_477

In vivo tissue specific modulation of rat insulin receptor gene expression in an experimental model of mineralocorticoid excess Campion, Javier Lahera, Vicente Cachofeiro, Victoria Maestro, Begoña dávila, norma Carranza, María del Carmen calle, consuelo insulin receptor gene expression mineralocorticoid-treated rats Insulin receptor (IR) gene expression at the mRNA level was investigated in hindlimb skeletal muscle, epididymal adipose tissue and in the liver of rats exposed to prolonged in vivo administration of deoxycorticosterone acetate (DOCA). Following treatment, plasma insulin levels were reduced while glucose levels increased compared to values in control rats. DOCA-treated animals showed an increase in blood pressure and a reduction in body weight. This treatment also induced hypokalemia and decreased plasma protein levels. Sodium levels were unaffected. Moreover, no differences in DNA and protein content or in the indicator of cell size (protein/DNA) were observed in the skeletal muscle or adipose tissue of animals. In contrast, there was a clear increase in the protein and DNA contents of the liver with no change in the indicator of cell size. Northern blot assays revealed 2 major IR mRNA species of approximately 9.5 and 7.5 Kb in the 3 tissues from control animals. DOCA treatment induced no change in the levels of either RNA species in skeletal muscle. However, a decrease of approximately 22% was detected in the levels of both species in adipose tissue whereas the liver showed an increase of 64%. These results provide the first evidence for an in vivo tissue-specific modulation of IR mRNA levels under experimental conditions of mineralocorticoid excess. 2026-06-18T11:18:15Z 2026-06-18T11:18:15Z 1998 1573-4919 0300-8177 https://hdl.handle.net/20.500.11984/14595 eng © 1998 Kluwer Academic Publishers. Springer Nature Link